Antigen

Antigen Class

TFs and others

Antigen

STAG2

Cell type

Cell type Class

Others

Cell type

Fibroblasts

NA

NA

Sample

source_name

ChIPseq_Fibroblast_SA2

antibody

SA2 (ab4463, Abcam)

treatment

NT

cell line/type

Fibroblast

Sequenced DNA Library

library_name

GSM6244248

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin immunoprecipitation was performed as previously described (Komata, M., et al., Chromatin immunoprecipitation protocol for mammalian cells. Methods Mol Biol, 2014. 1164: p. 33-8.). ChIP-seq libraries were prepared using NEBNext ChIP-seq Library Prep Master Mix Set for Illumina (New England BioLabs).

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

32785897

Reads aligned (%)

98.5

Duplicates removed (%)

5.3

Number of peaks

1382 (qval < 1E-05)

hg19

Number of total reads

32785897

Reads aligned (%)

97.4

Duplicates removed (%)

6.1

Number of peaks

1299 (qval < 1E-05)