Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
STAG2

Cell type

Cell type Class
Others
Cell type
Fibroblasts
NA
NA

Attributes by original data submitter

Sample

source_name
ChIPseq_Fibroblast_SA2
antibody
SA2 (ab4463, Abcam)
treatment
NT
cell line/type
Fibroblast

Sequenced DNA Library

library_name
GSM6244248
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation was performed as previously described (Komata, M., et al., Chromatin immunoprecipitation protocol for mammalian cells. Methods Mol Biol, 2014. 1164: p. 33-8.). ChIP-seq libraries were prepared using NEBNext ChIP-seq Library Prep Master Mix Set for Illumina (New England BioLabs).

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
32785897
Reads aligned (%)
98.5
Duplicates removed (%)
5.3
Number of peaks
1382 (qval < 1E-05)

hg19

Number of total reads
32785897
Reads aligned (%)
97.4
Duplicates removed (%)
6.1
Number of peaks
1299 (qval < 1E-05)

Base call quality data from DBCLS SRA